Hemolysis by Saponin and Sodium Taurocholate, with Special Reference to the Series of Ryvosh

نویسنده

  • J. Franklin Yeager
چکیده

1. The series of Ryvosh is obtained when hemolysis of the red cells of the animals concerned occurs with saponin as the lytic agent. 2. The series of Ryvosh is not obtained when R(infinity) is taken as the resistance constant and sodium taurocholate is used to hemolyse the cells of the same animals. 3. The hemolysin sodium taurocholate has been found to differ from saponin in that the time-dilution curves are found to approach their respective asymptotes with different values of kappa.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Kinetics of Progressive Reactions in Systems Containing Saponin, Digitonin, and Sodium Taurocholate

The relations between lysin concentration, percentage hemolysis at the moment at which the lysin concentration is reduced by dilution, and the amount of hemolysis which follows the dilution as a result of the reaction being "progressive" point to there being an "internal" phase at the red cell surfaces, in which the lysin is less affected by the dilution than in the system as a whole. A second ...

متن کامل

Volume Changes in Hemolytic Systems Containing Resorcinol, Taurocholate, and Saponin

Simultaneous measurement of hemolysis, the volume of the intact cells, and the K lost from the intact cells of systems containing resorcinol, sodium taurocholate, and saponin shows that the volume increases may be conspicuously small while the K losses are large, and that the volume increases are un-equal for equal K losses produced by different lysins. In higher concentrations of the same lysi...

متن کامل

The Effect of Hemolytic Substances on White Cell Respiration

Substances such as saponin, the bile salts, etc., which produce lysis of red cells also produce cytolysis of white cells from rabbit peritoneal exudates, the arbitrary criterion of their cytolytic effect being their ability to depress the O(2) consumption of the leucocytes. The amount of cytolysis increases regularly as the amount of the added lysin is increased, and sufficiently large quantiti...

متن کامل

The Prolytic Loss of K from Human Red Cells

The prolytic loss of K., i.e. the loss of K which takes place from red cells exposed to hypolytic concentrations of lysins, has been measured in systems containing distearyl lecithin, sodium taurocholate, sodium tetradecyl sulfate, saponin, and digitonin, by means of the flame photometer. The lysins are added in various concentrations to washed red cells from heparinized human blood, and the K ...

متن کامل

Comparative Kinetics of Hemolysis Induced by Bacterial and Other Hemolysins

A study has been made of the kinetics of lysis induced by various hemolytic agents. The course of bemolysis was followed by mixing lysin with washed human erythrocytes, removing samples from the mixture, and estimating colorimetrically the hemoglobin in the supernatant fluid of the centrifuged samples. Most of the curves (but not all of them, e.g. tyrocidine) obtained by plotting degree of hemo...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of General Physiology

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2003